ABSTRACT
Humoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)-2 is not fully understood yet but is a crucial factor of immune protection. The possibility of antibody cross-reactivity between SARS-CoV-2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n = 24 patient samples and n = 12 control samples were screened for antibodies against the entire SARS-CoV-2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43, and 229E. While widespread cross-reactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARS-CoV-2-derived peptides provided statistically significant discrimination between COVID-19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVID-19-specific diagnostic antibody tests.
Subject(s)
Antibodies, Viral/blood , COVID-19/diagnosis , Protein Array Analysis/methods , SARS-CoV-2/immunology , Viral Proteins/immunology , Adult , Aged , Amino Acid Sequence/genetics , Antibodies, Viral/immunology , Coronavirus 229E, Human/immunology , Coronavirus Nucleocapsid Proteins/immunology , Coronavirus OC43, Human/immunology , Cross Reactions/immunology , Diagnostic Tests, Routine , Female , Humans , Immunoglobulin G/blood , Immunoglobulin G/immunology , Male , Middle Aged , Middle East Respiratory Syndrome Coronavirus/immunology , Phosphoproteins/immunology , Proteome/immunology , Severe acute respiratory syndrome-related coronavirus/immunology , Spike Glycoprotein, Coronavirus/immunology , Young AdultABSTRACT
ABSTRACT The interconnected aspects of career and mental health posit career as a central feature of human functioning. A perceived lack of control within career and work exacerbates existing challenges associated with COVID-19. Focusing on enhancing agency within the realm of career and work is imperative within counselling. Cognitive Information Processing (CIP) accounts for the complexity of career concerns by enhancing agency in career decision making, with problem solving serving as a conduit between uncertainty and empowerment. The current state of employment, threats to agency within career development, and specific CIP-based interventions to enhance agency are discussed. (PsycInfo Database Record (c) 2021 APA, all rights reserved)